Antidiarrhoeal Effects of Hydromethanolic Leaves Extract of Ipomea asarifolia in Albino Rat Model

Main Article Content

A. N. Ukwuani-Kwaja
I. L. Yakubu
A. S. Mustapha
B. Makun

Abstract

Aim: To evaluate the antidiarrhoea effect of hydromethanolic leave extract of I. asarifolia (HLEIA) on castor oil-induced diarrhea.

Place and Duration of Study: Department of Biochemistry, Faculty of Life sciences, Kebbi State University of Science and Technology, Aliero, Kebbi state, Nigeria. P.M.B.1144. Kebbi State. Nigeria, between February 2015 and September 2016.

Methodology: In a continuous effort to search for bioactive agents from medicinal plants, the antidiarrhoea activity of I. asarifolia was investigated. The effect of hydromethanolic leave extract of I. asarifolia (HLEIA) on castor oil-induced diarrhoea, gastrointestinal transit and intestinal fluid accumulation (enteropooling) were assessed in albino rats. Qualitative phytochemical analysis was carried out using standard procedures while acute oral toxicity studies was determined using the staircase method.

Results: The phytochemical analysis showed the presence of alkaloid, terpenoid, tannin, saponin, phenols. The LD50 was estimated to be greater than 3000 mg/kg since there was no mortality recorded after 14 days of acute oral toxicity studies. Sub-chronic administration of graded doses (150 – 600 mg/kg) of HLEIA significantly (p<0.05) reduced diarrhoea episodes, decreased gastro intestinal movement and inhibited intestinal fluid accumulation compared to the control. The antidiarrhoea effect of treated group (600 mg/kg ) was comparable to that of the standard drug Loperamide.

Conclusion: The findings of the present study scientifically validate the use of I. asarifolia in the treatment of diarrhoea.

Keywords:
Gastro-intestinal transit, castor oil, enteropooling, loperamide, diarrhoea episodes

Article Details

How to Cite
Ukwuani-Kwaja, A. N., Yakubu, I. L., Mustapha, A. S., & Makun, B. (2019). Antidiarrhoeal Effects of Hydromethanolic Leaves Extract of Ipomea asarifolia in Albino Rat Model. Journal of Complementary and Alternative Medical Research, 7(4), 1-7. https://doi.org/10.9734/jocamr/2019/v7i430106
Section
Original Research Article

References

Bibitha BJ, Jisha VK, Salitha CV, Mohan S, Valsa AK. Antibacterial activity of different plant extracts, Short communication. Indian J Microbiol. 2002;42:361–363.

Karou D, Savadogo A, Canini A, Yameogo S, Montesano JS, Colizzi V, Traore AS. Antibacterial activity of alkaloids from Sida acuta. Afri J Biotechnol. 2006;5(2):195-200.

Okigbo RN, Eme UE, Ogbogu S. Biodiversity and conservation of medicinal and aromatic plants in Africa. Biotechnol. Mol. Biol. Rev. 2008;3(6):127-134.

Cheikhyoussef A, Mapaure I, Shapi M. The use of some Indigenous Plants for Medicinal and other Purposes by Local Communities in Namibia with Emphasis on Oshikoto Region: A Review. Res J Med Plants. 2011;5(4):406-419.
DOI: 10.3923/rjmp.2011.406.419

De Silva T. Industrial utilization of medicinal plants in developing countries. In: Medicinal plants for forest conservation and good health. Food and Agriculture Organization of the United Nation, Rome. 2014;34 – 44.
[ISSN 1020-3370]

Jegede IA, Nwinyi FC, Ibrahim J, Ughabe G, Zarma SD, Kunle OF. Investigation of phytochemical, anti-inflammatory and anti-nociceptive properties of Ipomoea asarifolia leaves. J Med Plant Res. 2009; 3(3):160 – 164.

WHO. World health organization fact sheet: Diarrhoeal disease; 2017.
Available:http://www.who.int/news-room/fact-sheets/detail/diarrhoeal-disease

Abdullahi AL, Agho MO, Amos S, Gamaniel KS, Wambebe C. Antidiarrhoeal activity of the aqueous extract of Terminalia avicennoides roots. Phytother. Res. 2001;15(5):431-434.
DOI: http://dx.doi.org/10.1002/ptr.860

UNICEF/WHO. United Nations Children’s Fund and World Health Organization, ‘WHO/UNICEF Joint Statement: Diarrhoea: Why children are still dying and what can be done; 2009.
Available:http://apps.who.int/iris/bitstream/handle/10665/44174/9789241598415_eng.pdf?sequence=1

Brijesh S, Tetali P, Birdi TJ. Study of effect of anti-diarrheal medicinal plants on enteropathogenic Escherichia coli induced interleukin-8 secretion by intestinal epithelial cells. Altern Med Stud. 2011; 1(16):64-69.
DOI: 10.4081/ams.2011.e16

Yakubu MT, Opakunle FK, Salimon SS, Ajiboye TO, Bamisaye FA, Quadri AL. Antidiarrheal activity of aqueous leaf extract of Ceratotheca sesamoides in rats. Bangladesh J Pharmacol. 2012;7(1):14-20. DOI: 10.3329/bjp.v7i1.9789

Trease GE, Evans WC. Trease and Evans Pharmacognosy. A physician guide to Herbal medicine. 15th Edition: Ballere tindal London, U.K; 1989.

Dixon WJ. Staircase bioassay; the up and down method. Nuero Sci Biobehave Rev. 1991;15(1):47-50.
DOI:https://doi.org/10.1016/S0149- 7634(05)80090-9

Pazhani GP, Subramanian N, Arunchalam G, Hemalatha S, Ravichandran V. Antidiarrheal potential of Elephantopus scaber Linn leaf extract. Ind Drugs. 2001; 38 (5):269-271.

Awouters F, Niemegeers CJE, Lenaerts FM, Janseen PAJ. Delay of castor oil diarrhea in rats; A new way to evaluate inhibitors of prostaglandin biosynthesis. J Pharmacol. 1978;30(1):41-45.
DOI:https://doi.org/10.1111/j.20427158.1978.tb 13150.x

Robert A, Nezamis JE, Lancaster C, Hanchar AJ, Klepper MS. Enteropooling assay, a test for diarrhea produced by prostaglandins. Prostagladins. 1976;11(5): 809-828.

OECD. Acute oral toxicity - up and down procedure. Guidelines for testing of chemicals. 425:1-26.

Atta AH, Mounier M. Evaluation of some medicinal plants extracts for antidiarrhoeal activity. Phytother. Res. 2005;19(6):481-485.
DOI: https://doi.org/10.1002/ptr.1639

Gutiérrez SP, Sánchez MAZ, González CP, García LA. Antidiarrhoeal activity of different plants used in traditional medicine. Afr J Biotechnol. 2007;6(25): 2988-2994.

Oh SW, Ryu BH. Experimental Studies on the Antidiarrheal Effects of Anjang-san. Korean J. Orient. Med. 2011;32(6): 54-66.

Sharma DK, Gupta VK, KumarS, Joshi V, Mandal RSK, Prakash AGB, Singh M. Evaluation of antidiarrheal activity of ethanolic extract of Holarrhena antidysenterica seeds in rats. Vet World. 2015;8(12):1392–1395.
Available:http://doi.org/10.14202/vetworld.2015.1392-1395

Schiller LR, Santa Ana CA, Morawski SG, Fordtran JS. Mechanism of the antidiarrheal effect of loperamide. Gastroenterology.1984;86(6):1475-1480.

Hardman JG, Limbird LE. Drugs affecting gastrointestinal function. In: The pharmacological basis of therapeutics. 10th Edn. Goodman and Gilman’s Edn. New York. Macgraw Hill. 2001;1023-1024.

Vieira C, Evangelista S, Cirillo R, Lippi A, Maggi CA, Manzini S. Effect of ricinoleic acid in acute and subchronic experimental models of inflammation. Mediators Inflamm. 2000;9(5):223–228.
DOI: 10.1080/09629350020025737

Rouf AS, Islam MS, Rahman MT. Evaluation of anti-diarrhoeal activity of Rumex maritimus roots. J. Ethnopharmacol. 2003;84(2-3):307–310.
DOI:https://doi.org/10.1016/S0378-8741(02)00326-4

Palombo EA. Phytochemicals from traditional medicinal plants used in the treatment of diarrhoea: Modes of action and effects on intestinal function. Phytother Res. 2006;20(9):717-24.
DOI: https://doi.org/10.1002/ptr.1907

Loganga OA, Vercruysse A, Foriers A. Contribution to the ethanobotanical, Phytochemical and pharmacology studies of traditionally used medicinal plant in the treatment of dysentery and diarrhoeal in Lomela area, Democratic Republic of Congo (DRC). J. Ethnopharmacol. 2000; 71(3):411-423.
DOI: https://doi.org/ 10.10 16/S0378-8741(00)00167-7

Das SK, Samantaray D, Thatoi H. Ethnomedicinal, Antimicrobial and Antidiarrhoeal Studies on the Mangrove Plants of the Genus Xylocarpus: A Mini Review. J Bioanal Biomed. 2014;S12: 004.
DOI: 10.4172/1948-593X.S12-004

Otshudi AL, Foriers A, Vercrysse A, Van Zeebroek A, Lauwers S. In-vitro antimicrobial activity of six medicinal plants traditionally used for the treatment of dysentery and diarrhea in democratic Republic Congo (DRC). Phytomed. 2000; 7(2):167-172.
DOI:https://doi.org/10.1016/S0944-7113(00)80090-2

Geyid A, Abebe D, Debella A, Makonnen Z, Aberra F, Teka F, Kebede T, Urga K, Yersaw K, Biza T, Mariam BH, Guta M. Screening of some medicinal plants of Ethiopia for their anti-microbial properties and chemical profiles. J Ethnopharmacol. 2005;97(3):421-7.
DOI:https://doi.org/10.1016/j.jep.2004.08.021

Balaji G, Chalamaiah M, Ramesh B, Amarnath YR. Antidiarrhoeal activity of ethanol and aqueous extracts of Carum copticum seeds in experimental rats. Asian Pac J Trop Biomed. 2012;2(2):1151-1155. DOI: https://doi.org/10.1016/S2221-1691(12)60376-1