Protective Effect of Acanthospermum hispidum DC (Asteraceae) Extracts against Diethylnitrosamine Induced Hepatocellular Damage
Jotham Yhi-Pênê N’do *
Laboratory of Biochemistry and Applied Chemistry (LABIOCA), University of Ouaga I Pr Joseph KI-ZERBO, 03 BP 848 Ouagadougou 03, Burkina Faso.
Adama Hilou
Laboratory of Biochemistry and Applied Chemistry (LABIOCA), University of Ouaga I Pr Joseph KI-ZERBO, 03 BP 848 Ouagadougou 03, Burkina Faso.
Dramane Pare
Laboratory of Biochemistry and Applied Chemistry (LABIOCA), University of Ouaga I Pr Joseph KI-ZERBO, 03 BP 848 Ouagadougou 03, Burkina Faso.
Ernest Nogma Sombie
Laboratory of Biochemistry and Applied Chemistry (LABIOCA), University of Ouaga I Pr Joseph KI-ZERBO, 03 BP 848 Ouagadougou 03, Burkina Faso.
Tata Kadiatou Traore
Doctoral School of Health, University of Ouaga I Pr Joseph KI-ZERBO, P.O.Box 7021, 03 BP 848 Ouagadougou 03, Burkina Faso.
Noufou Ouedraogo
Doctoral School of Health, University of Ouaga I Pr Joseph KI-ZERBO, P.O.Box 7021, 03 BP 848 Ouagadougou 03, Burkina Faso and Institute for Research in Health Sciences (IRSS/CNRST), Department of Medicine and Traditional Pharmacopoeia (MEPHATRA-PH), 03 BP 7192 Ouagadougou 03, Burkina Faso.
André Tibiri
Laboratory of Biochemistry and Applied Chemistry (LABIOCA), University of Ouaga I Pr Joseph KI-ZERBO, 03 BP 848 Ouagadougou 03, Burkina Faso and Institute for Research in Health Sciences (IRSS/CNRST), Department of Medicine and Traditional Pharmacopoeia (MEPHATRA-PH), 03 BP 7192 Ouagadougou 03, Burkina Faso.
Latifou Lagnika
Unit of Biochemistry and Molecular Biology, Laboratory of Biochemistry and Bioactive Natural Substances, Faculty of Science and Technology, University of Abomey-Calavi, 04 BP 0320 Cotonou, Republic of Benin.
*Author to whom correspondence should be addressed.
Abstract
Aims: Liver diseases are a major public health concern in the world. About 120 million cases of infectious HBV (the half of the world cases) are registered in sub-Saharan Africa. Burkina Faso is a country with a high endemicity of viral hepatitis. These diseases cause serious damage to the liver.
Study Design: This study aimed to assess the non-toxicity and the good hepatoprotective activities of Acanthospermum hispidum extracts.
Place and Duration of Study: Animal model tests and in vitro hepatoprotection tests were conducted from June 2017 to March 2018.
Methodology: For in vivo hepatoprotective potential study, rats were pretreated with Acanthospermum hispidum extracts at different doses and intoxicated with diethylnitrosamine. For the evaluation of the toxicity of the extracts, the OCED protocols served as a benchmark.
Results: Ethanolic extract at a dose of 250 mg/kg body weight protected the livers of animals against hepatotoxin attacks. The safety of this extract was verified at a single dose of 2000 mg/kg and repeated doses of 300 mg/kg. Approaches on the action mechanism of the extracts were assessed using the in vitro tests.
Conclusion: Acanthospermum hispidum extracts at the doses used showed hepatoprotective potential. The in vitro activities through the inhibition of the activity of certain enzymes involved in the physiology of hepatitis made it possible to carry out approaches on the mechanisms of action of the extracts. The proven safety combined with the hepatoprotection of the extracts justifies their use against hepatic infections.
Keywords: Acanthospermum hispidum, hepatitis, hepatoprotection, histopathology, diethylnitrosamine, Burkina Faso.