Triterpenoids from Poria cocos and Their Potential In vitro Hepatoprotective Effects
Yihai Wang
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China
Guoqiong Wang
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China
Xiaomin Yi
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China
Xiangjiu He *
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China
*Author to whom correspondence should be addressed.
Abstract
Aims: To isolate triterpenoids from the surface layer of Poria cocos and evaluate their hepatoprotective activities in vitro.
Study Design: Triterpenoids were isolated and identified from the surface layer of Poria cocos. Their effects on alcohol induced-liver injury models were investigated in vitro.
Place and Duration of Study: School of Pharmacy, Guangdong Pharmaceutical University, between September 2013 and December 2013.
Methodology: Triterpenoids were purified by Silica gel, ODS as well as HPLC chromatography and characterized by MS, 1D and 2D NMR. The hepatoprotective effects of the isolated compounds against alcohol induced-liver injury were investigated in L-02 cell line.
Results: Eight triterpenoids were isolated and identified from the surface layer of Poria cocos (1–8), Compounds 2, 3, 6 showed potent protective effects against ethanol-induced injury on L-02 cells.
Conclusion: This study suggests that triterpenoids from the surface of Poria cocos could protect L-02 cells from death induced by alcohol and suitable for alcohol induced-liver injury patients as medicine or functional food, which would be a new candidate for the treatment of alcoholic liver disease.
Keywords: Poria cocos, triterpenoids, hepatoprotective, L-02