Antiproliferative Activity of Ethanol Extract of Boswellia dalzielii (Hutch) Stem Bark in Breast Cancer Cell Line
A. P. Otitoju *
Department of Biochemistry, Faculty of Medical Sciences University of Jos, Jos, Nigeria. and Medical Biotechnology Department, National Biotechnology Development Agency, Abuja, Nigeria. and Clinical Pharmacology Department, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi-Mumbai, 410210, India.
I. Y. Longdet
Department of Biochemistry, Faculty of Medical Sciences University of Jos, Jos, Nigeria.
T. E. Alemika
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Jos, Jos, Nigeria.
V. P. Gota
Clinical Pharmacology Department, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi-Mumbai, 410210, India.
*Author to whom correspondence should be addressed.
Abstract
Aim and Objectives: The present study aimed to examine the antiproliferative activity of ethanol extract derived from the Boswellia dalzielii stem bark in breast carcinoma.
Methodology: Ethanolic extract of stem bark of Boswellia dalzielii were prepared. Antiproliferative activity was assessed in estrogen receptor (ER)-negative breast carcinoma (MDA-MB-231) cells by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay.
Results: Obtained results in 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay indicated that ethanol extract of Boswellia dalzielii stem bark showed significant antiproliferative activity in MDA-MB-231 cells in a dose-dependent manner. IC50 of Boswellia dalzielii stem bark ethanol extract in MDA-MB231 was 98. 12 μg/mL.
Conclusion: The results obtained suggest that Boswellia dalzielii stem bark extract possesses significant antiproliferative potential which could be mediated by the chemical constituents present in the plant. However, further research needs to be carried out to determine the effect of the extract on the cell cycle and also to determine the type of cell death produced by the extract.
Keywords: Antiproliferative activity, MDA-MB231, Boswellia dalzielii.