Ameliorative Effects of Ascorbic Acid and Allium sativum (Garlic) Ethanol Extract on Renal Parenchyma of Gentamicin-induced Nephropathic Rats

Main Article Content

Dayo Rotimi Omotoso
Joy Motunrayo Olajumoke

Abstract

To assess ameliorative effects of Ascorbic acid (AA) and Allium sativum ethanol extract (ASEE) on renal parenchyma of gentamicin-induced nephropathic rats.

Thirty Wistar rats (weighing between 180-205 g) were randomly divided into five groups (A-E).  These include Group A administered with 0.9% Normal Saline (0.5 ml/kg body weight (b.w.)), Group B administered with gentamicin (GM, 200 mg/kg b.w.) intraperitoneally (i.p.), Group C administered with GM (200 mg/kg b.w.) i.p. and AA (200 mg/kg b.w.) orally, Group D administered with GM (200 mg/kg b.w.) i.p. and ASEE (200 mg/kg b.w.) orally and Group E administered with GM (200 mg/kg b.w.) i.p. and AA (200 mg/kg b.w.) orally and ASEE (200 mg/kg b.w.) orally. All administrations were done once daily for a period of ten (10) days. The body weight of study animals was recorded at the beginning and end of study period. After the study period, renal tissue of study animals was harvested, weighed, processed, stained using H & E technique. Stained sections were examined under microscope for histopathological changes within the renal parenchyma and were scored using image-J software.

The results of this study showed that exposure to GM results into significant (P < 0.05) reduction in body and renal tissue weight. However, therapeutic exposure to AA and ASEE either as individual or combined treatment regimen culminated into relatively null body and renal tissue weight loss among treatment groups C-E. In addition, exposure to GM precipitates prominent histopathological changes within renal parenchyma of study animals.  As observed with body and renal tissue weight changes, treatment with AA and ASEE also comparatively ameliorate GM-induced nephropathy within renal parenchyma of study animals in treatment groups.

The findings of this study therefore showed that AA and ASEE exhibit ameliorative effect on the renal parenchyma of gentamicin-induced nephropathic rats either as distinct or combined treatment regimen.

Keywords:
Ascorbic acid, Allium sativum, gentamicin-induced nephropathy, renal parenchyma, wistar rats.

Article Details

How to Cite
Omotoso, D. R., & Olajumoke, J. M. (2020). Ameliorative Effects of Ascorbic Acid and Allium sativum (Garlic) Ethanol Extract on Renal Parenchyma of Gentamicin-induced Nephropathic Rats. Journal of Complementary and Alternative Medical Research, 9(4), 1-8. https://doi.org/10.9734/jocamr/2020/v9i430146
Section
Original Research Article

References

Sembulingam K, Sembulingam P. Essentials of medical physiology. Fifth edition. Jaypee Brothers Medical Publishers Ltd, St Louis, USA. 2010;288.

Young B, O’Dowd G, Woodford P. Wheater’s functional histology: A text and colour atlas. Sixth Edition. Elsevier Churchill Livingstone PA, USA. 2014;292.

Teslariu O, Pasca AS, Mititelu-Tartau L, Schiriac CE, Gales C, Saftencu PM, Nechifor M. The protective effects of Zinc in experimental gentamicin induced acute renal failure in rats. J Physiol Pharmacol. 2016;67(5):751-757.

Alarifi S, Al-Doaiss A, Alkahtani S, Al-Farraj, Al-Eissa MS, Al-Dahmash B, Al-Yahya H, Mubarak M. Blood chemical changes and renal histological alterations induced by gentamicin in rats. Saudi J Biol Sci 2012;19(1):103-110.
DOI: 10.1016/j.sjbs.2011.11.002

Omotoso DR, Lawal OS, Olatomide OD, Okojie IG. Nephroprotective effect of Cissampelos owariensis extract on renal histomorphology of Wistar rats during exposure to carbon tetrachloride-induced nephropathy. Asian J Biol. 2019;8(4): 1-10.
DOI: 10.9734/AJOB/2019/v8i430071

Lakshmi BVS, Sudhakar M. Protective effect of Zingiber officinale on gentamicin-induced nephrotoxicity in rats. Int J Pharmacol. 2010;6(1):58-62.

Ozbeck E, Cekmen M, Ilbey YO, Simsek A, Polat EC, Somay A. Atorvastatin prevents gentamicin-induced renal damage in rats through the inhibition of p38-MAPK and NK-kappa B pathways. Ren Fail. 2009;31:382-392.

Morales AI, Detaille D, Prieto M, Puente A, Briones E, Arevalo M, Leverve X, Lopez-Novoa JM, El-Mir M. Metformin prevents experimental gentamicin-induced nephropathy by a mitochondria-dependent pathway. Kidney Int. 2010;77(10):861-869.
DOI: 10.1038/ki.2010.11

Padayatty SJ, Katz A, Wang Y, Eck P, Kwon O, Lee J, Chen S, Corpe C. Vitamin C as an antioxidant: Evaluation of its role in disease prevention. J Am Coll Nutr. 2003;22(1):18-35.

Ibegbu AO, Animoku AA, Ayuba M, Brosu D, Adamu SA, Akpulu P, Hamman WO, Umana UE, Musa SA. Effect of ascorbic acid on mercuric chloride-induced changes on the cerebral cortex of Wistar rats. Afr J Cell Pathology. 2013;1:23-29.

Acharya C, Thakar H, Vajpeyee SK. A study of oxidative stress in gentamicin induced nephrotoxicity and effect of antioxidant Vitamin C in wistar rats. Nat J Physiol Pharm Pharmacol. 2013;3(1):14-20.
DOI: 10.5455/njppp.2013.3.14-20

Okwuonu UC, Omotoso DR, Bienonwu EO, Adagbonyin O, Dappa J. Histomorphological profile of liver and kidney tissues of albino Wistar rats following exposure to cadmium-induced damage and ascorbic acid supplementation. Acad Anat Int. 2020;6(1): 6(1):15-19.
DOI: 10.21276/aanat.2020.6.1.5

Mohandas R, Paramesha S, Suchetha KN, Damodara GKM, Vidhya GG. Effect of Garlic on total antioxidants in Alloxan induced Diabetic mellitus rats. Int J Biomed Adv Res. 2011;2(9):317-328.

Chinnala KM, Jayagar PP, Motta G, Adusumilli RC, Elsani MM. Evaluation of hepatoprotective activity of Allium sativum extract in thioacetamide-induced hepatotoxicity in albino Wistar rats. Am J Res Med Sci. 2018;3(2):48-53.

Omotoso DR, Akinola AO, Daramola OO. Possible stimulatory role of CTLA-4 gene in anti-diabetic activity of ethanolic extracts of Allium sativum (Garlic) using rat model. J Med Allied Sci. 2019;9(2):90-95.
DOI: 10.5455/jmas.49716

Adeneye AA, Olagunju JO. Protective effect of oral ascorbic acid (Vitamin C) on acetaminophen-induced renal injury in rats. Afr J Biomed Res. 2009;12(1):55-61.

Khan MR, Badar I, Siddiquah A. Prevention of hepatorenal toxicity with Sonchus asper in gentamicin treated rats. BMC Compl Alt Med. 2011;11:113.

Fischer AH, Jacobson KA, Rose J, Zeller R. Haematoxylin and eosin staining of tissue and cell sections. Cold Spring Harbor Protocols; Cold Spring Harbor, NY, USA: Cold Spring Harbor Press; 2008.

Yarijani ZM, Najafi H, Hamid Madani S. Protective effect of crocin on gentamicin-induced nephrotoxicity in rats. Iran J Basic Med Sci. 2016:19:337-43.

Varatharajan R, Jun LH, Kai TZ, Jian LW, Anburaj J, Vijayan V. Morphological and morphometric study of Edaravone in gentamicin-induced nephrotoxicity in Sprague Dawley Rats. J Young Pharm 2017;9(1):31-35.
DOI: 10.5530/jyp.2017.9.6

Mazzon E, Britti D, De Sarro A, Caputi AP & Cuzzocrea S. Effect of N-acetylcysteine on gentamicin-mediated nephropathy in rats. Eur J Pharmacol. 2001;424:75-83.

Maldonado PD, Barrera D, Rivero I, Mata R, Medina-Campos ON, Hernandez-Pando R, Pedraza-Chaverri J. Antioxidant S-allylcysteine prevents gentamicin-induced oxidative stress and renal damage. Free Radic Biol Med. 2003;35:317–324.

Balakumar P, Rohilla A, Thangathirupathi A. Gentamicin-induced nephrotoxicity: Do we have a promising therapeutic approach to blunt it? Pharmacol Res. 2010;62:179–186.

Lopez-Novoa JM, Quiros Y, Vicente L, Morales AI, Lopez-Hernandez FJ. New insights into the mechanism of aminoglycoside nephrotoxicity: An integrative point of view. Kidney Int. 2011; 79:33-45.

Dawud FA, Eze ED, Ardja AA, Isa AS, Jimoh A, Bashiru M, Malgwi IS. Ameliorative effects of vitamin C and zinc in alloxan-induced diabetes and oxidative stress in Wistar rats. Curr Res J Bio Sci. 2012;4(2):123-129.

Oguntibeju OO. The biochemical, physiological and therapeutic roles of ascorbic acid. Afr J Biotechnol. 2008;7(25): 4700-4705.

Afifi OM, Embaby AS. Histological study on the protective role of Ascorbic acid on cadmium induced cerebral cortical neurotoxicity in adult male Albino rat. J Microsc Ultastruct. 2016;4:36-45.
DOI: 10.1016/j.jmau.2015.10.001

Kadkhodaee M, Khastar H, Faghihi M,Ghaznavi R, Zahmatkesh M. Effects of co-supplementation of vitamins E and C on gentamicin-induced nephrotoxicity in rat. Exp Physiol. 2005;90(4):571-576.
DOI: 10.1113/expphysiol.2004.029728

Ariharan VN, Kalirajan K, Meena Devi VN, Prasad NP. An exotic fruit which forms the new natural source for vitamin-C.Rasayan J Chem. 2012;5:356-359.

Kadi IE, Dahdouh F. Vitamin C pretreatment protects from nickel-induced acute nephrotoxicity in mice. Arh Hig Rada Toksikol. 2016;67:210-215

Mahesar H, Bhutto MA, Khand AA, Najero NT. Garlic used as an alternative medicine to control diabetic mellitus in alloxan-induced male rabbits. Pak J Physiol. 2010; 6(1):39-41.

Hussein ZM. Effect of garlic on dyslipidemic patients with diabetes mellitus (Type 2). Diyala J Med. 2014;6(1):100-104.

Thomson M, Al-Amin ZM, Al-Qattan KK, Shaban LH, Ali M. Anti-diabetic and hypolipidaemic properties of garlic (Allium sativum) in streptozotocin-induced diabetic rats Int J Diabetes & Metabolism. 2007;15: 108-115.

Anwar MM, Meki AR. Oxidative stress in streptozotocin-induced diabetic rats: effects of garlic oil and melatonin. Comp Biochem Physiol A Mol Integr Physiol. 2003;135:539-547.