Antinociceptive and Anti-inflammatory Properties of Triumfetta cordifolia A. Rich. (Tiliaceae) Root Methanol/Dichloromethane Extract
Chameni Nkouankam Maeva Jenna
Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P.O. Box 2701, Douala, Cameroon.
Nguemfo Edwige Laure
Department of Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P.O. Box 2701, Douala, Cameroon.
Hzounda Jean Baptiste
Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P.O. Box 2701, Douala, Cameroon.
Ngene Jean Pierre
Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P.O. Box 2701, Douala, Cameroon.
Nguemnang Tchatchouang Russelle Camelie
Department of Biology and Physiology of Animal Organisms, Faculty of Sciences, University of Douala, P.O. Box 24157 Douala, Cameroon.
Nsegbe Annick Christianne
Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P.O. Box 2701, Douala, Cameroon.
Kuinze Kojap Augustine
Department of Biology and Physiology of Animal Organisms, Faculty of Sciences, University of Douala, P.O. Box 24157 Douala, Cameroon.
Mbogbe Nadia Elise
Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P.O. Box 2701, Douala, Cameroon.
Songue Sylvie Pascale
Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P.O. Box 2701, Douala, Cameroon.
Sone Enone Bertin
Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P.O. Box 2701, Douala, Cameroon.
Francois Eya’ane Meva *
Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P.O. Box 2701, Douala, Cameroon.
*Author to whom correspondence should be addressed.
Abstract
Objective: Triumfetta cordifolia is a terrestrial plant of the Tiliaceae family used traditionally for the treatment of many diseases. Different parts are traditionally used for the treatment of diseases that cause pain and inflammation. This study aims to evaluate the antinociceptive and anti-inflammatory activities of the methanol/dichloromethane extract of T. cordifolia roots in animal models.
Methodology: Phytochemical screening and the toxicity study of methanol/dichloromethane extract of Triumfetta cordifolia roots were performed. Antinociceptive activity was evaluated using acetic acid and formalin tests. In vitro anti-inflammatory activity was evaluated using the bovine serum albumin (BSA) denaturation method and In vivo on carrageenan-induced oedema. The extract was given orally at doses of 50, 100 and 150 mg/kg of body weight.
Results: The phytochemical analysis revealed the presence of alkaloids, saponins, flavonoïds, polyphenols, reducing sugars, tannins, coumarins and steroids. No signs of toxicity were observed after 14 days experiment. Regarding antinociceptive activity, Triumfetta cordifolia (200 mg/kg) has decreased significantly (P ˂ 0.0001) the number of abdominal contortions and also significantly inhibited the formalin-induced neurogenic pain at the dose of 50 mg/kg body weight. In anti-inflammatory tests, the extract inhibited oedema (P˂ 0,001) at 50mg/kg at the fourth hour and also protected the serum albumin bovine denaturation (P <0.05) at a concentration of 50 μg/ml.
Conclusion: The results obtained indicate that the methanol/dichloromethane extract is without acute toxicity and has peripheral and central analgesic properties, as well as anti-inflammatory properties In vivo and In vitro.
Keywords: Triumfetta cordifolia, methanol/dichloromethane extract, analgesic, anti-inflammatory, phytochemical screening, toxicity