Journal of Complementary and Alternative Medical Research

Background: Coffee is a globally consumed beverage, and substantial interest is being shown in its biological effects. It contains both irritant and protective agents.

Objective: This experiment aimed to evaluate the impact of roasted Coffea arabica/C. arabica (arabica coffee) and Coffea canephora/C. canephora (robusta coffee) on the histopathological alterations in the gastric tissue of Mus musculus.

Methods: A laboratory-based experimental design, utilizing a control group setup, was employed for this study. Thirty mice were randomly assigned to one of three groups: a control group (K) receiving standard chow and distilled water, a group administered C. arabica powder at a dose of 0.1 grams dissolved in 1.8 ml of distilled water per day, gavage for 7 consecutive days, and a group receiving an equivalent dose of C. canephora powder via the same administration protocol. Gastric tissues were processed using standard paraffin embedding techniques, followed by Hematoxylin and Eosin (HE) staining for histological examination. The histopathological assessment of gastric tissue was conducted to evaluate the extent and severity of morphological damage, utilizing the Wattimena scoring system. Statistical analysis was performed using the Kruskal-Wallis test within the IBM SPSS Statistics 25 software suite.

Results: The results indicated that neither roasted C. arabica nor roasted C. canephora induced significant histopathological changes in the gasters of the mice (Mus musculus). Additionally, no statistically significant differences were observed between the effects of both coffee powders on the gastric histopathology.