Journal of Complementary and Alternative Medical Research

Aims: To analyze the chemicals composition of methanolic extracts of leaf and stem of M. minuta collected from Uppur Village, Tamilnadu, India by GC-MS.

Study Design: Experimental study.

Methodology: The Methanolic extracts were prepared and concentrated at 40°C using hot air oven. The concentrated methanolic extracts were subjected to GC-MS analysis using the instrument Perkin Elmer Clarus 500.

Results: The methanolic extract of leaf of M. minuta showed the presence of 36 phyto compounds including n-Hexadecanoic acid (44.41%) (C₁₆H₃₂O₂); (Z)6,(Z)9-Pentadecadien-1-ol (35.49%) (C₁₅H₂₈O); Phytol (5.10%) (C₂₀H₄₀O); 2-Cyclohexane-1-one,4-hydroxy-3,5,6-trimethyl-4  (3-oxo-1-butenyl)   (2.25%) (C₁₃H₁₈O₃); 9,12,15-Octadecatrienoic acid (Z,Z,Z) (2.0%) (C₁₈H₃₀O₂); 3,7,11,15-Tetramethyl-2-hexadecan-1-ol (1.99%) (C₂₀H₄₀O) and Benzofuran, 2,3-dihydro- (1.1%) (C₈H₈O). The methanolic extract of stem of M. minuta showed the presence of 27 bioactive compounds including 1,19-Eicosadiene (40.94%) (C₂₀H₃₈); n-Hexadecanoic acid (20.13%) (C₁₆H₃₂O₂); 2(R)3(S)-1,2,3,4-Butanetetrol (16.31%) (C₄H₁₀O₄); Glycerin (6.70%) (C₃H₈O₃); Octadecanoic acid (4.24%) (C₁₈H₃₆O₂); Benzofuran, 2,3-dihydro- (1.73%) (C₈H₈O); 3,7,11,15 – Tetramethyl-2 hexane decen-1-ol (1.55%) (C₂₀H₄₀O); n-Hexadecanoic acid methyl ester (1.15%) (C₁₇H₃₄O₂) and 10- Octadecenoic acid methyl ester (1.42%) (C₁₉H₃₆O₂).

Conclusion: The results of the present study concluded that the presence of various phytocompounds in leaf and stem of M. minuta. Therefore, plants are the rich sources of chemicals but largely unknown and unexplored. So, it is our hope that this study will encourage further research on isolation and purification of therapeutically important phytocompounds from M. minuta