Journal of Complementary and Alternative Medical Research <p style="text-align: justify;"><strong>Journal of Complementary and Alternative Medical Research (ISSN: 2456-6276)</strong> aims to publish high quality papers (<a href="/index.php/JOCAMR/general-guideline-for-authors">Click here for Types of paper</a>) in the areas of Complementary, Alternative and Integrative medical research. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer reviewed, open access INTERNATIONAL journal.</p> SCIENCEDOMAIN international en-US Journal of Complementary and Alternative Medical Research 2456-6276 Mangifera indica (Mango) Bark Therapy Potentiates Wound Healing in Diabetic Rats <p><strong>Objective: </strong>One of the debilitating complications of diabetes mellitus is chronic foot ulcer. <em>Mangifera indica</em> (Mango) is a naturally occurring and widely cultivated plant with many health benefits attributable to its parts. The wound healing potential of its bark in adult diabetic rats was investigated.</p> <p><strong>Methods: </strong>The excised wounds of diabetic adult female wistar rats in groups of six were dressed with mango bark powder (MPD), mango bark ethanolic extract (MED), sofratulle (SD) and normal saline (ND) daily till healed. The corresponding control groups were MPC, MEC, SC and NC respectively. Every three days, the mean wound contraction rates were calculated from the measured wound areas. Granulation tissue was biopsied from an animal per group on day 3, 6 and 9 for histopathological evaluation and after healing, the scars of the remaining animals were biopsied for histology.</p> <p><strong>Results:</strong> The <em>M. indica </em>bark powder contained greater quantity of coumarins than the ethanolic extract; with terpenoids and steroids detected only in the powder. The MPD group had the highest mean wound contraction rates for the specified period. The mean wound contraction rates for the MPC group were significantly higher than those of the MED. The granulation tissues of the MPD and MPC groups had similar microscopic features to those of MEC, MED, SC, SD, NC and ND. Microscopy of the wound scars showed stratified squamous epithelia with abundant collagen fibres and blood vessels with dermal appendages seen in some of the groups.</p> <p><strong>Conclusion:</strong> Findings from this study showed that the <em>M. indica</em> bark.</p> Rotimi Sunday Ajani Opeyemi Jeremiah Olateju ##submission.copyrightStatement## 2020-06-13 2020-06-13 1 13 10.9734/jocamr/2020/v10i130152 Anxiolytic Mechanism(S) and Corticosterone-Attenuating Effect of Hydroalcoholic Leaf Extract of Tapinanthus globiferus Mistletoe Growing on Azadirachta indica Tree <p>Similar pharmacodynamic mechanism(s) often underlie drug actions and toxicities of anxiolytic agents and medicinal extracts. Extracts of <em>Tapinanthus globiferus</em> and related plant species have been reported with anxiolytic activities. But mechanistic evaluations on these plant extracts are few. This study investigated the anxiolytic mechanism(s), including the corticosterone-attenuating effect, of hydroalcoholic <em>Tapinanthus globiferus</em> (HATG) leaf extract harvested from <em>Azadirachta indica</em> host tree in the mouse elevated zero-maze and restraint-induced acute stress paradigms using per cent open segment time (%OST) and brain/plasma corticosterone levels as endpoints, respectively. The results show that anxiolytic activity (%OST) of 150 mg/kg HATG leaf extract was reversed by pretreatment with 5 mg/kg caffeine (HATG alone, 10.90±1.73;HATG+Caffeine, 8.66±1.74), 2 mg/kg methysergide (MTD) (HATG alone, 98.70±14.98; HATG+MTD, 74.20±10.82) and 5 mg yohimbine (HATG alone, 120.10±10.72; HATG+Yohimine, 78.44±13.92) but not by 0.5 m/kg atropine (HATG alone, 104.60±25.31; HATG+Atropine, 105.40±11.85), 0.5 mg/kg flumazenil (HATG alone,80.27±9.69; HATG+Flumazenil, 80.75±10.19), 2 mg/kg cyproheptadine (HATG alone, 88.67±16.44; HATG+Cyproheptadine, 92.11±12.58), 0.2 mg/kg haloperidol (HATG alone, 74.11±17.33; HATG+Haloperidol, 94.00±32.54) and 5 mg/kg naloxone (HATG alone, 94.30±10.84; HATG+Naloxone, 95.30±6.86). The results also indicate HATG leaf extract (at 50, 150, 500 and 1500 mg/kg) caused largely dose-dependent and significant (<em>p</em>&lt;0.05) attenuations in brain/plasma corticosterone levels (5.64±0.66/3.91±0.44,3.78±0.39/3.39±0.38, 4.26±0.34/3.22±0.18 and 2.74±0.51/2.74±0.22), respectively, in extract- compared to distilled water- (5.93±0.60/4.56±0.37) and diazepam-treated (2.34±0.19/2.44±0.29) mice subjected to restraint-induced acute stress. These findings suggest anxiolytic mechanism(s) of the extract may involve its interactions with the adenosine, non-5HT2 serotonin, alpha (α)2 receptors and the hypothalamus-pituitary-adrenal (HPA) axis. This study may constitute the first mechanistic and corticosterone modulation report on the extracts of this parasitic medicinal plant and may benefit from confirmatory radio-labelled binding assays in subsequent studies.</p> A. M. Umarudeen M. G. Magaji ##submission.copyrightStatement## 2020-06-23 2020-06-23 14 23 10.9734/jocamr/2020/v10i130153 Preliminary Phytochemical Screening of Five Plants as Possible Antileishmaniasis Control Agent <p>Leishmaniasis is a major public health problem globally and manifests in three clinical forms including visceral cutaneous and mucocutaneous. Visceral leishmaniasis is fatal if left untreated for a period of 2 years, while cutaneous leishmaniasis cause crusted papules or ulcers on exposed skin. Plant families containing active compounds against other protozoan diseases may be suitable against leishmania parasites. This study report the compounds extracted from five plants (<em>Olea europaea, Kigelia Africana, Terminalia mollis, Croton macrostachyus and Bridella micrantha </em>extracts). The plants were collected from Baringo County in Kenya and authenticated at the National Museums of Kenya (Department of Botany). The plant samples were dried, pulverized into fine powders and extracted using methanol at the Center for Traditional Medicine and Drugs Research, KEMRI. The plant extracts contained varying amounts of phytochemical compounds such as tannins, phenols, flavonoids, steroids, alkaloids, saponins, anthraquinone, cardiac glycoside, polyphenols, cumarins, anthocyanins, trepenoids, glycosides and triterpenoids. The presence of tannins, flavanoids, alkaloids and saponins with known biological activities offer opportunity to test these compounds against leishmania parasites.</p> Mukhwana Dennis Wafula ##submission.copyrightStatement## 2020-07-06 2020-07-06 24 30 10.9734/jocamr/2020/v10i130154